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受託試験

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To the persons who are involved pharmaceutical companies, food  companies and cosmetic companies

WE, ANBAS (AUTONOMIC NERVE BASED SCIENCES) CO. LTD, ACCEPT ENTRUSTED STUDIES TO IDENTIFY FUNCTIONS OF DRUGS, FOODS, COSMETICS, ESSENTIAL OILS AND THEIR ORIGINAL MATERIALS BY ELECTROPHYSIOLOGICAL DETERMINATION OF CHANGES IN ACTIVITIES OF AUTONOMIC NERVES INNERVATING EACH ORGANS/TISSUES AFTER ADMINISTERING THEM PER OS, INTO THE STOMACH AND INTESTINE, INJECTION INTO THE VEIN, APPLICTIONS ON THE SKIN AND INHALATION.

Our company is the only one company in the world which is able to determine changes in activities of autonomic nerves innervating each organ and tissue of rats and mice by electrophysiological method. This technology was originally established by late Professor Akira Niijima (Professor Emeritus of Niigata University, 1921-2015). However, this technique is very difficult to be mastered. Under the direction and training by Professor Akira Niijima, we could fortunately obtain this technique.

Thus, would you please let us examine functions of drugs, foods, cosmetics, essential oils  and their possible original materials by this technology?  
Katsuya Nagai, MD PhD
Professor Emeritus Osaka University
President of ANBAS Corporation


If you have any questions or inquiries for us, please send them through our contact form below.

TEL: +81-6-6486-1080
FAX: +81-6-6486-1081

Please refer published articles of us below:

  • A Niijima and K Nagai (2003) Effect of olfactory stimulaton with flavor of grapefruit oil and lemon oil on the activity of sympathetic branch in the white adipose tissue of the epidermis. Exp. Biol. Med. (Maywood) 228: 1190-1192.
  • M Tanida, A Niijima, Y Fukuda, H Sawai, N Tsuruoka, J Shen, S Yamda, K Kiso,
K Nagai (2005) Dose-dependent effects of L-carnosine on the renal sympathetic nerve
and blood pressure in urethane-anesthetized rats. Am. J. Physiol. 288: R447-R455.
  • M Tanida, T Yamano, K Maeda, N Okumura, Y Fukushima, K Nagai (2005) Effects of
intraduodenal injection of Lactobacillus Johnsonii La1 on renal sympathetic nerve activity and blood pressure in urethane-anesthetized rats. Neurosci. Lett. 389:109-114.
  • H Taniguchi, M Tanida, N Okumura, J Hamada, S Sano, K Nagai, (2006) Regulation of Sympathetic and Parasympathetic Nerve Activities by BIT/SHPS-1. Neurosci. Lett. 398: 102-106.
  • M Tanida, J Shen, Y Horii, M Matsuda, S Kihara, T Funahashi, I Shimomura, H SawaI, Y Fukuda, Y Matsuzawa, K Nagai (2007) Effects of adiponectin on the renal sympathetic nerve activity and blood pressure in rats. Exp. Biol. Med. (Maywood) 232: 390-397.
  • M Tanida, N Tsuruoka, J Shen, Y Kiso, K Nagai (2008) Effects of oolong tea on renal sympathetic nerve activity and spontaneous hypertension in rats. Metabolism 57: 526-534.
  • T Nakamura, M Tanida, A Niijima, J Shen, K Nagai (2007) Auditory stimulation affects renal sympathetic nerve activity and blood pressure in rats. Neurosci. Lett 416: 107-112.
  • J Shen, H Nakamura, Y Fujisaki, M Tanida, Y Horii, R Fuyuki, H Takumi, K Shiraishi, T Kometani, K Nagai (2009) Effects of 4G-alpha-glucopyranosyl hesperidin on brown fat adipose tissue- and cutaneous-sympathetic nerve activity and peripheral body temperature.Neurosci. Lett. 461: 30-35.
  • Y Horii, Y Nikaido, K Nagai, T Nakashima (2010) Exposure to TMT odor affects adrenal sympathetic nerve activity and behavioral consequences in rats. Behav. Brain Res. 214: 317-322.
  • M Tanida, K. Nagai (2011) (Review) Electrophysiological analysis of the mechanism of autonomic action by Lactobacilli. Biosci. Microflora 30: 99-109.
  • Y Horii, M Tanida, J Shen, Y Fujisaki, R Fuyuki, K Hashimoto, T Nakashima, K Nagai. (2011) Effects of urea-containing cream on cutaneous arterial sympathetic nerve activity, blood flow and water evaporation. Skin Res Technol 17:75-81.
  • K Nagai,M Tanida, A Niijima, N Tsuruoka, Y Kiso, Y Horii, J Shen, N Okumura (2012) (Review) Role of L-carnosine in the control of blood glucose, blood pressure, thermogenesis, and lipolysis by autonomic nerves in rats: involvement of the circadian clock and histamine. Amino Acids 43: 97-109.
  • Y Horii, J Shen, Y Fujisaki, K Yoshida, K Nagai (2012) Effects of L-carnosine on splenic sympathetic nerve activity and tumor proliferation.Neurosci. Lett. 510: 1-5.
  • Y Beppu, H Komura, T Izumo, Y Horii, J Shen, M Tanida, T Nakasima, N Tsuruoka,
K Nagai (2012) Identificaton of 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one isolated from <i>Lactobacillus pentosus</i> strain S-PT84 culture supernatants as compound that stimulates autonomic nerve activities in rats. J. Agric Food Chem 60: 11044-11049
  • Y Horii, K Nagai, T Nakashima (2013) Order of exposure to pleasant and unpleasant odors affects autonomic nervous system response. Behav Brain Res. 243: 109-117
  • T Izumo, T Maekawa, Y Horii, Y Fujisaki, M Ida, Y Furukawa, Y Ono, Y Kiso,
Y Kitagawa, H Shibata, K Nagai (2013) The effect of the sympathetic nervous system on splenic natural killer cell activity in mice administered the Lactobacillus pentosus strain S-PT84. Neuroreport. 24(17):988-991.
  • Y Horii, H Kaneda, Y Fujisaki, R Fuyuki, Y Nakakita, T Shigyo, K Nagai (2014) Effect of heat-killed Lactobacillus brevis SBC8803 on cutaneous arterial sympathetic nerve activity, cutaneous blood flow and transepidermal water loss in rats. J. Appl. Microbiol.116: 1274-1281.
  • K Nagai, A Niijima, Y Horii, J Shen, M Tanida (2014) (Review) Olfactory stimulatory with grapefruit and lavender oils change autonomic nerve activity and physiological function. Auton Neurosci. 185: 29-35.
  • Y Horii, Y Nakakita, Y Fujisaki, H Kaneda, T Shigyo, K Nagai (2014) Intraduodenal injection of heat-killed lactobacillus brevis SBC8803 modulates autonomic neurotransmission in the gut: Involvement of vagal afferents and serotonin receptors. Curr Top Nutraceutical Res 13: 69-74.
  • Y Horii, T Mizoguchi, Yi Fujisaki, R Fuyuki, K Nagai (2014) Effects of Chlorella Pyrenoidosa on cutaneous arterial sympathetic nerve activity, cutaneous blood flow and transepidermal water loss in rats, Curr Top Nutraceutical Res 12: 107-114, 2014.
  • Y Horii, K Maeda, J Shen, Y Fujisaki, K Nagai(2015) Effect of grapefruit and lavender essential oil scents on pancreatic sympathetic nerve activity and plasma glucose in rats. Flavour Fragr. J. 30:282-287.
  • Y Horii, Y Nakakita, Y Misonou, T Nakamura, K Nagai (2015) The serotonin receptor mediates changes in autonomic neurotransmission and gastrointestinal transit induced by heat-killed Lactobacillus brevis SBC8803. Beneficial Microbes. 6:799-806.
  • Y Horii, Y Fujisaki, Y Misonou, K Nagai (2017) Effects of chlorella pyrenoidosa on splenic sympathetic nerve activity and tumors induced by human colon cancer cells. Curr. Top. Nutraceut. Res. 15: 81-86, 2017/07/26
  • H Miyazaki, T Nakamura, K Ohki, K Nagai (2017) Effects of the bioactive peptides Ile-Pro-Pro and Val-Pro-Pro upon autonomic neurotransmission and blood pressure in spontaneously hypertensive rats. Autonomic Neuroscience: Basic and Clinical 208: 88-92.
  • Ohara, T Fukuda, Y Ishida, C Takahashi, R Ohya, M Katayama, K Uchida,
M Tominaga, K Nagai (2017) b-Eudesmol, and oxygenized sesquiterpene, stimulates appetite via TRPA1 and the autonomyc nervous system. Sci. Rep. 2017 Nov 17;7(1):15785. doi: 10.1038/s41598-017-16150-6.
  • L Zhou, m Ohata, C Owashi, K Nagai, K Arihara (2018) Odors generated from the Maillard reaction affect autonomic nervous activity and decrease blood pressure through the olfactory system. J Sci Food Agric. 98: 923-927 2017 Jul 12. doi: 10.1002/jsfa.8538. [Epub ahead of print]
  • K Nagai, Y Horii, Y Fujisaki, R Fuyuki, Y Misonou (2018) Effects of olfactory stimulation with scents of grapefruit and lavender essential oils on the skeletal muscle sympathetic nerve and muscle blood flow in rats. Flavour Fragr J 33: 135-143
  • K Ohara, M Katayama, K Nagai (2018) b-Eudesmol, an oxygenized sesquiterpene, affects efferent adrenal sympathetic nerve activity via transient receptor potential ankyrin 1 in rats. Neurosci Lett. 684: 18-24, 2018 Sep 25;684:18-24. doi: 10.1016/j.neulet.2018.06.057. Epub 2018 Jun 30.
  • Y Horii, Y ujisaki, R Fuyuki, Y Misonou, K Nagai (2019) Effect of Lactobacillus delbrueckii LAB4 on hepatic and pancreatic sympathetic nerve activity and blood glucose levels in rats. Benef Microbes.10: 293-300
  • Horii, Y. Fujisaki, Y. Misonou, R. Fuyuki, K. Nagai (2020) Effects of ethyl hexanoate on activities of sympathetic nerves innervating the brown and white adipose tissues, body temperature, and plasma fatty acids. Neurosci.Lett.737, 15 Oct 2020, 135319
  • K Egawa, Y Horii, Y Misonou, I Yamasaki, D Takemoto, Y Ono, T Rogi, H Shibata, K Nagai K. (2020) Sesame lingans increase sympathetic nerve activity and blood flow in rat skeletal muscle. Physiol, Res. 69: 253-260,2020 [https://doi.org/10.33549/physiolres.934277]